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Chromatography Affinity

Affinity chromatography is a chromatographic separation method for isolating an analyte from a solution of different substances. The prerequisite is that a suitable ligand (binding partner) to the analyte of interest (protein) is available. It is one of the most powerful separation methods. Primarily, affinity chromatography is used to purify and concentrate a substance from a mixture into a buffer solution or to reduce a quantity of substance in a mixture. Affinity chromatography is also commonly used to determine which biological compounds bind to a particular substance or to purify and concentrate an enzyme solution.

Chromatography Affinity

Affinity chromatography is a chromatographic separation method for isolating an analyte from a solution of different substances. The prerequisite is that a suitable ligand (binding partner) to the analyte of interest (protein) is available. It is one of the most powerful separation methods.
Primarily,…

Affinity chromatography is a chromatographic separation method for isolating an analyte from a solution of different substances. The prerequisite is that a…


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