Kits cDNA Synthesis

cDNA is DNA that is synthesized from RNA by means of the enzyme reverse transcriptase (RT). The enzyme reverse transcriptase can be used to produce the complementary cDNA from RNA. Like other polymerases, this specific, RNA-dependent DNA polymerase requires a primer (a short, complementary DNA segment) for synthesis, which binds to the RNA. The product is now a cDNA strand hybridized to the original RNA strand. The latter can now be degraded with the RNase H enzyme. In further processing, the complementary DNA strand to the already existing cDNA single strand is now synthesized by means of a DNA-dependent DNA polymerase (via a primer). The result is a double-stranded cDNA. Since the original template was a processed RNA (which, among other things, has already undergone splicing), there are no introns on the cDNA, in contrast to natural eukaryotic DNAs.