Column chromatography in chemistry is a chromatographic technique used to isolate a single chemical compound from a mixture. Chromatography can be used to separate substances based on the differential adsorption of compounds onto the adsorbent (stationary phase); the compounds move through the column at different speeds so that they can be separated into fractions. The stationary phase is contained in a cylindrical tube – the separation column. This so-called packing material can fill the entire cavity of the tube (packed column) or be applied only as a thin layer on the inner surface (capillary column).
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