Flow Cytometer
Flow cytometers analyze individual cells using light scattering and fluorescence. They enable detailed cell characterization for research and diagnostics.
Product
Flow Cytometer
Flow cytometers analyze individual cells using light scattering and fluorescence. They enable detailed cell characterization for research and diagnostics.
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Blanc-Labo SA
Milian SA
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Guide and selection support
Flow cytometers are used for rapid analysis and quantification of cells based on size, internal structure, and fluorescent labeling. They find application in areas such as cell biology, immunology, and clinical diagnostics.
When choosing a flow cytometer, factors such as the number of measurement channels, type of fluorescence detection, and required throughput are crucial. Compatibility with fluorescence markers and ease of operation are also important considerations.
LabFinder assists by offering clear categorization and technical information, helping you find the right device configuration for specific laboratory needs.
Application and Benefits
Flow cytometers enable rapid, multiparametric analysis of cells and particles that are passed individually past detectors in a liquid stream. These instruments measure light scattering and fluorescence signals to determine cell size, granularity, surface antigens, and intracellular markers. Applications include cell research, immune status determination, cancer diagnostics, and quality control of biological samples.
Selection Criteria
Important criteria when selecting a flow cytometer include the number and type of available detection channels, the sensitivity of fluorescence detection, and compatibility with various fluorophores. Other aspects are sample throughput, automation options, data analysis software features, and device ergonomics. Depending on the application, specific requirements for cell sorting or multicolor analyses may be relevant.
Variants and Measurement Principles
Fundamentally, flow cytometry is based on the principle of guiding cells individually through a laser beam and detecting light scattering as well as fluorescence emissions. Variants differ in the number of lasers used, measurement channels, and fluorescence markers. Instruments with sorting functionality (e.g., Fluorescence-Activated Cell Sorting, FACS) specifically separate labeled cells based on the measured parameters.
Calibration and Maintenance
Regular calibration with standard particles is essential to ensure measurement accuracy and reproducibility. Maintenance includes checking and cleaning the sample fluidics, light sources, and detectors. Careful operation and continuous monitoring of system performance help maintain stable laboratory results.
Limitations of Application
Flow cytometry requires well-prepared cell samples, as contamination or aggregates can impair measurement accuracy. Some cell types or markers may not be optimally detected, for example when a fluorophore lacks stability. Additionally, high investment and operator expertise are necessary, limiting use to specialized laboratory environments.
Search Terms and Related Terms
Synonyms and related terms for flow cytometer include: Flow Cytometer, Flow-Zytometer, cell sorter, FACS instrument, fluorescence flow cytometer, and cell measurement device. Relevant search terms are flow cytometry, cell analysis, fluorescence labeling, cell sorting, and cell structure measurement.
Also known as
Cell Cytometer
Cell Measurement Device
Cell Sorter
Cytometer
FACS instrument
Flow-Zytometer
Flowcytometer
Flow Cytometry Instrument
Fluorescence Cytometer
Fluorescence Flow Cytometer
Related categories
Analytical instruments Clinical diagnostics - General
Assays Cell Counting
Assays Fluorescence Quantification
Cell Analyzer
Cell Counter
Cell Sorter
Centrifuges - General purpose centrifuge
Cryoscope
Fluorescence measurement
Lysing Matrix Tubes
Magnetic Cell Separation
Single-Cell Multiomics Systems
Suitable brands
Frequently asked questions
How does a flow cytometer work?
A flow cytometer passes cells individually through a laser beam and measures scattered light signals and fluorescence emissions to provide information about cell size, structure, and markers.
What types of flow cytometers are there?
Generally, instruments vary in the number of lasers and detection channels; some models also offer a sorting function (FACS) for targeted cell separation.
What are important selection criteria for flow cytometers?
Considerations include the number and type of fluorescence channels, sample throughput, compatibility with markers, software support and ease of use, and, if required, cell sorting options.
What are the main applications of flow cytometers?
They are used for rapid multiparametric analysis of cell populations in research and diagnostics, such as immunoassays, cancer research, or cell counting.
What are the limitations of flow cytometry?
Limitations include poor sample preparation, unsuitable fluorophores, the need for trained personnel, and higher investment costs.
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