Chromatography HPLC

High performance liquid chromatography – also called high pressure liquid chromatography in the early days of this technique – is an analytical method in chemistry. HPLC is a liquid chromatography technique that not only separates substances, but also identifies and quantifies them (determines the exact concentration) using standards. Unlike gas chromatography, which is a very good separation method for vaporizable substances, HPLC can also be used to analyze non-volatile substances. HPLC can also be used preparatively.