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Microtome for histological sections

Microtomes for histological sections produce precise, thin slices of tissue for microscopic examination. They are essential instruments in histological sample preparation.

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Microtome for histological sections

Microtomes for histological sections produce precise, thin slices of tissue for microscopic examination. They are essential instruments in histological sample preparation.

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Highlights

All Highlights

Guide and selection support

Microtomes are used to prepare ultrathin sections from biological tissue that are necessary for microscopic analysis. The section thickness typically lies in the micrometer range, enabling detailed studies of tissue structures.

When selecting a microtome, section thickness, cutting technique, and the degree of automation are important factors to meet the specific requirements of histological laboratory work. Different types of microtomes offer various cutting modes for different sample types.

LabFinder assists in orientation and comparison of various microtome devices by providing clear, detailed product descriptions and technical specifications. This makes it easy to find and procure suitable models.

Applications and Benefits

Microtomes are precision cutting devices used to prepare thin sections from biological tissue for histological examination. They are indispensable in pathology, histology, and related biomedical fields to create microscopy slides that allow for detailed tissue assessment.

The section thickness can range from approximately 0.1 µm to 100 µm, which is much thinner than a human hair. This enables microtomes to produce section samples suitable for highly detailed morphological analysis.

Selection Criteria

Key decision criteria when choosing a microtome include the possible section thickness, the type of cutting technique (manual or motorized), and the type of specimen (e.g., paraffin-embedded tissue, frozen sections). Additional factors are ergonomics, user comfort, and compatibility with appropriate consumables.

Variants and Cutting Principles

Microtomes are available in various forms, including rotary microtomes that utilize a rotating knife movement, and cryostat microtomes designed for frozen tissue sections. Vibrating microtomes, which use an oscillating blade, are a specialized alternative but are considered a separate device category.

Calibration and Maintenance

Regular maintenance and precise adjustment are essential for consistent section quality. This includes sharpening the blades, monitoring section thickness, and cleaning the mechanical components. Manufacturer instructions regarding care support the long-term operational reliability of the devices.

Limits of Application

Microtomes are suitable for cuttable, well-prepared tissue samples; very hard or brittle materials can present problems. They are specifically designed for the production of precision tissue sections and are not intended for making other types of samples.

Search Terms and Related Concepts

Synonyms and related search terms include histological microtome, tissue sectioning device, sample slicer, fine sectioning machine for histology, microtome apparatus, rotary microtome, and cryostat microtome. These terms help with product and information searches.

Also known as

Device for histological sectioning Fine sectioning machine histological Histological microtome Histological sectioning machine Microtome apparatus Microtome device Microtome for tissue sections Microtomes Microtome system Sample slicer

Frequently asked questions

How does a microtome for histological sections work?

A microtome uses a very sharp blade to cut thin slices of tissue that are prepared for microscopic examination. The tissue is precisely guided and repositioned after each cutting movement.

What types of microtomes are available for histological applications?

Common types include rotary microtomes for paraffin-embedded sections, cryostat microtomes for frozen samples, and vibrating microtomes that use oscillating blades. The choice depends on the sample type and application.

What are the key criteria when selecting a microtome?

Important criteria include adjustable section thickness, degree of automation, ergonomics, compatibility with blades and specimens, and flexibility for various tissue types.

How are microtomes maintained and calibrated?

The blades should be regularly sharpened and cleaned, and the section thickness is adjusted manually or automatically depending on the device. Proper cleaning and checking of the mechanism ensure consistent quality.

What are the limits of using microtomes?

Microtomes are suitable for well-prepared, cuttable tissue samples. Very hard, brittle, or unprepared specimens are more difficult to cut and may damage the blades.

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