DPBS – Dubelcco’s Phosphat Buffered Saline – DPBS ohne Kalzium ohne Magnesium – 100 ml

Theoretical pH : 7.3 0.3
Osmolality : 290 mOsm/kg 10%
Colour : colourless, clear solution
Storage conditions : Room temperature
Shelf life : 48 months
Sterility tests :
– Bacteria in aerobic and anaerobic conditions
– Fungi and yeasts
Endotoxin : < 1 EU/ml
Composition : Displayed on website ; also available on request
Recommended use :
– Respect storage conditions of the product
– Do not use the product after its expiry date
– Store product in an area protected from light (not necessary for saline solutions).
– Manipulate the product in aseptic conditions (e.g. : under laminar air flow)
– Wear clothes adapted to the manipulation of the product to avoid contamination (e.g. :
gloves, mask, hygiene cap, overall…)
The product is intended to be used in vitro, in laboratory only. Do not use it in therapy, human
or veterinary applications.
Application :
Dulbecco’s Phosphate Buffered Saline (DPBS) is intended for use in the maintenance of
mammalian cells where a chemically defined, balanced salt solution provides an environment
that will maintain the structural and physiological integrity of cells in vitro. DPBS is
commonly used in cell enumeration as a diluent, for rinsing cells and as a buffer in many
chromatographic procedures. DPBS is also used to wash and resuspend cells during the
dissociation process. This product has neither calcium nor magnesium ions in its composition
so it doesn’t inhibit the activity of the trypsin.
Utilisation :
Supplements, such as antibiotics, should be added as sterile supplements to the buffer
solution. Storage conditions and shelf-life of the supplemented product will be affected by the
nature of the supplements.
Signs of Deterioration :
Buffer solution should be clear and free of particulate and flocculent material.
Do not use if buffer solution is cloudy or contains precipitate.
Other evidence of deterioration may include degradation of physical or performance
characteristics.