qPCRBIO SyGreen Mix is designed for fast, highly sensitive and reproducible real-time PCR.
The antibody-mediated hot start system prevents the formation of primer dimers and non-specific products leading to greatly improved reaction sensitivity and specificity.
qPCRBIO SyGreen Mix combines a proprietary non-inhibiting intercalating dye (detected in the FAM channel) with the latest advances in polymerase technology and buffer chemistry to give you fast, highly sensitive and reproducible real-time PCR.
qPCRBIO SyGreen Mix can be used to reliably quantify any DNA template including genomic, cDNA and viral sequences, and is able to detect extremely low copy number targets with the highest efficiency.
Rapid extension rate for early Ct values Market-leading sensitivity – increased limit of detection Specific amplification from complex templates including GC-rich sequences Non-PCR inhibiting intercalating dye Also available as an easy-to-see blue mix Compatible with all standard and fast cycling real-time instruments
The 2x mix uses a proprietary intercalating dye that does not inhibit PCR, unlike other popular fluorescent dyes. The same developments that improve the sensitivity and consistency of qPCRBIO SyGreen Mix in standard cycling conditions also allow for industry-leading performance in fast and ultra fast cycling conditions.
Applications
Absolute quantification
qPCRBIO SyGreen Mix is designed for fast, highly sensitive and reproducible real-time PCR.
The antibody-mediated hot start system prevents the formation of primer dimers and non-specific products leading to greatly improved reaction sensitivity and specificity.
qPCRBIO SyGreen Mix combines a proprietary non-inhibiting intercalating dye (detected in the FAM channel) with the latest advances in polymerase technology and buffer chemistry to give you fast, highly sensitive and reproducible real-time PCR.
qPCRBIO SyGreen Mix can be used to reliably quantify any DNA template including genomic, cDNA and viral sequences, and is able to detect extremely low copy number targets with the highest efficiency.
Rapid extension rate for early Ct values
Market-leading sensitivity – increased limit of detection
Specific amplification from complex templates including GC-rich sequences
Non-PCR inhibiting intercalating dye
Also available as an easy-to-see blue mix
Compatible with all standard and fast cycling real-time instruments
The 2x mix uses a proprietary intercalating dye that does not inhibit PCR, unlike other popular fluorescent dyes. The same developments that improve the sensitivity and consistency of qPCRBIO SyGreen Mix in standard cycling conditions also allow for industry-leading performance in fast and ultra fast cycling conditions.
Applications
Absolute quantification
Relative gene expression analysis
High-throughput qPCR from genomic, cDNA and viral sequences
Detection of extremely low copy number targets
Crude sample PCR
The kit should be stored between -30°C and -15°C. Avoid prolonged exposure to light. If stored correctly the kit will retain full activity for 12 months. The kit can be stored at 4°C for 1 month. The kit can go through 30 freeze/thaw cycles with no loss of activity.
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