Live Cell Imaging
Live cell imaging enables time-resolved observation of living cells under the microscope. It is used to investigate cell dynamics and biological processes in vitro.
Product
Live Cell Imaging
Live cell imaging enables time-resolved observation of living cells under the microscope. It is used to investigate cell dynamics and biological processes in vitro.
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LaboTechSystems LTS AG
Milian SA
Tecnilabo
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Guide and selection support
Live cell imaging is used in cell biology and molecular research to analyze living cells over time and gain a better understanding of biological processes. Specialized microscopy systems are often employed, utilizing fluorescence labeling and time-lapse techniques. The selection of appropriate equipment depends on the type of samples, desired imaging parameters, and available fluorescence techniques. LabFinder provides a structured overview of products for live cell imaging, allowing users to efficiently find suitable microscopy technologies and accessories to meet their research needs.
Applications and Benefits
Live cell imaging encompasses the microscopic examination of living cells over defined observation periods. The aim is to analyze cell functions, cell division, cell dynamics, and signal transduction in vitro. Applications are found in cell biology, molecular biology, and pharmacology, for example to study cellular reactions to drugs or environmental influences. The technique supports time-resolved acquisition of dynamic processes that cannot be captured with static images.
Selection Criteria
For live cell imaging, the performance of the microscope, the sensitivity of the detectors, and the integration of fluorescence filter sets are crucial. The type of fluorophores used, the required temporal resolution, as well as options for temperature and CO2 control of the cell samples are important. Compatibility with cell culture vessels and control over image acquisition (e.g., time-lapse mode) also influence the choice.
Variants and Measurement Principles
Typical approaches are based on fluorescence or brightfield microscopy, complemented by confocal or slit-lamp-based techniques. Fluorescent labels make it possible to specifically visualize cellular structures or molecules. Time-lapse microscopy acquires image series at defined intervals to dynamically document changes.
Calibration and Maintenance
Regular calibration of optical components, inspection of fluorescence filters, and camera maintenance are necessary to ensure consistent image quality and meaningful results. Cleaning of objectives and care of sample holders also form part of regular maintenance.
Limitations of Application
The quality of live cell imaging is limited by phototoxicity from intense illumination, photochemical damage to fluorophores, as well as cell selection and sample preparation. Some methods also require a compromise between spatial and temporal resolution.
Search Terms and Related Terms
Synonyms for live cell imaging include live-cell microscopy, live-cell imaging, time-lapse microscopy, fluorescence live imaging, or cell life cycle microscopy. Relevant keywords are cell culture, fluorescence, cell dynamics, time-resolved cell imaging, and live-cell microscopy.
Also known as
Cell dynamics microscopy
Fluorescence live imaging
Human and animal cell imaging
Live-cell-microscopy
Live-cell imaging
Live-cell microscopy
Live cell image analysis
Live cell monitoring
Live cell observation
Time-lapse microscopy of living cells
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Frequently asked questions
How does live cell imaging work?
Live cell imaging uses time-resolved microscopy, often with fluorescent labeling, to observe living cells over longer periods and visualize dynamic processes.
Which types of microscopy are used for live cell imaging?
Fluorescence microscopes and time-lapse microscopes are mainly used, supplemented by brightfield or confocal microscopy, depending on the application and required image quality.
What are important criteria when selecting equipment for live cell imaging?
Key criteria include fluorescence sensitivity, temporal resolution, compatibility with cell culture plates or vessels, as well as the ability to control environmental conditions and image acquisition.
What limitations does live cell imaging have?
Limitations arise from possible phototoxicity, limited fluorophore lifespan, and the need to compromise between temporal and spatial resolution.
How is live cell imaging maintained and calibrated?
Regular calibration of optical components, checking of filters, cleaning of objectives, and maintenance of the camera are required to ensure image quality.
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