When searchers choose their serum an important factor that should be taken into consideration is thesource, which also emphasises the traceability of the serum. Our system of vertical integration allows us to be certain of the origins and traceability of our Calf and Donor Calf Serum. Each manufactured batch is rigorously controlled, from the collection of serum and throughout all stages of its treatment and production through to final packaging on our premises. Clearline Calf Serum is derived from clotted whole blood collected from calf with cleaned and disinfected equipment. Clearline Donor Calf Serum is derived from clotted whole blood aseptically collected from donor calf via the vein before freezing. The serum is collected or imported and treated in agreement with the European regulations.
Filtration : Final Filter Size : 0.2μm
Sterility : All sera are tested for the absence of aerobic and anaerobic bacteria, fungi, yeast and Mycoplasma. The sterility test is based on the European Pharmacopoeia requirements. The sera are tested for the absence of Mycoplasma by culture.
Virus Tested : All of our sera are tested for: – Bovine Viral Diarrhoea (BVD)
When searchers choose their serum an important factor that
should be taken into consideration is thesource,
which also emphasises the traceability of the serum.
Our system of vertical integration allows us to be certain of the origins
and traceability of our Calf and Donor Calf Serum.
Each manufactured batch is rigorously controlled, from the collection of serum
and throughout all stages of its treatment and production through
to final packaging on our premises.
Clearline Calf Serum is derived from clotted whole blood collected from calf with cleaned and disinfected equipment. Clearline Donor Calf Serum is derived from clotted whole blood aseptically collected from donor calf via the vein before freezing.
The serum is collected or imported and treated in agreement with the European regulations.
Filtration :
Final Filter Size : 0.2μm
Sterility :
All sera are tested for the absence of aerobic and anaerobic bacteria, fungi, yeast and Mycoplasma.
The sterility test is based on the European Pharmacopoeia requirements.
The sera are tested for the absence of Mycoplasma by culture.
Virus Tested :
All of our sera are tested for:
– Bovine Viral Diarrhoea (BVD)
– Cytopathogenic agents e.g. Infectious Bovine Rhinotracheitis (IBR) / BHV-1
– Hemadsorbing agents e.g. Parainfluenza Type 3 (Pl3)
Sera are tested for the absence of the indicated viruses by inoculation to permissive cells. The
revelation is made by immunofluorescence for pestiviruses. Cytopathogenic agents and hemadsorbing
agents are detected by microscopic observations.
The antibodies are tested by an Elisa method.
Endotoxin :
All sera are tested to determine the levels of endotoxins. BioWest carries out a chromokinetic
quantitative test, according to the method D of the European Pharmacopoeia.
The endotoxin reagent is standardized against the US reference endotoxin.
Haemoglobin :
The haemoglobin level is measured by spectrophotometer.
Osmolality :
Determined by a lowered freezing temperature. The osmometer is calibrated against standard solutions
Cell Culture :
Biological performance is assessed using cell culture medium supplemented with the serum being
tested. During the test period, cultures are examined microscopically for any morphological abnormalities
that may indicate toxic components in the serum.
Technical data sheet
Ref : FT.S0400an
Page : 2/2
Version date : 20/07/17
Cell Lines Tested :
The following cell lines are tested with the serum:
HELA -Cancer Cell/Human.
L929 -Fibroblast-Mouse/ As Macrophage
SP2/0-AG14 -Mouse/Lymphoma
Total Protein :
Determined by Biuret Colorimetry.
Country of Origin :
The country in which the serum was taken from the donor/animal.
BioWest sera are sourced from the following countries
Cat N° S0400 France or Canada
Data Sheet [PDF]
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