Electrophoresis System
Electrophoresis systems separate DNA, RNA, and proteins by molecular size using gel electrophoresis. They are available in horizontal and vertical formats.
Product
Electrophoresis System
Electrophoresis systems separate DNA, RNA, and proteins by molecular size using gel electrophoresis. They are available in horizontal and vertical formats.
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Witec AG
Axon Lab AG
Milian SA
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Guide and selection support
Electrophoresis systems are used in laboratories for the analysis and separation of nucleic acids and proteins. Typical applications include molecular biology studies and quality control.
The selection of an electrophoresis system depends on the sample type, throughput requirements, and preferred gel orientation (horizontal or vertical). Other factors include the number of gels processed simultaneously and compatibility with detection methods.
LabFinder offers an overview of practical electrophoresis systems to facilitate targeted searching for suitable instruments for various analyses and laboratory needs.
Application and Benefits
Electrophoresis systems are used to separate DNA, RNA, and proteins based on their size. After applying a uniform electric charge, the molecules migrate through a porous gel, with smaller molecules moving faster than larger ones. This method is central to molecular biology, biochemistry, and diagnostics for analyzing the structure and purity of biomolecules.
Selection Criteria
Choosing a suitable electrophoresis system depends on several factors: the type of biomolecules to be separated (DNA, RNA, proteins), the desired gel orientation (horizontal or vertical), and the required sample throughput capacity. It is also important to consider system compatibility with existing analytical devices and ease of use, such as regarding gel changes and control of running parameters.
Variants and Measurement Principles
Electrophoresis systems are available as horizontal or vertical units. Horizontal systems are often used for DNA and RNA analysis, while vertical systems are more common in protein analysis. The measurement principle is based on the migration of charged molecules in an electric field through a gel matrix, which acts as a molecular sieve.
Calibration and Maintenance
To maintain separation quality, electrophoresis systems should be regularly checked for functional reliability, particularly power supply and cooling systems. Cleaning the gel units after each use and examining connections contribute to long service life. Calibration includes checking running speeds and gel temperatures.
Limits of Application
Electrophoresis systems are designed for separation of molecules within a certain size range and are not suitable for highly complex mixtures without further purification. Separation results may depend on gel type and running conditions. Additionally, the method is more time-consuming than automated alternatives such as capillary electrophoresis.
Search Terms and Related Terms
Synonyms and related terms include electrophoresis device, electrophoresis apparatus, gel electrophoresis system, molecular biology instruments, and electrophoresis separation system. Searches may also use terms such as DNA separation, protein separation, and horizontal or vertical electrophoresis.
Also known as
electrophoresis apparatus
electrophoresis device
electrophoresis equipment
electrophoresis pipeline
electrophoresis platform
electrophoresis technique
electrophoresis unit
gel electrophoresis system
laboratory electrophoresis device
Related categories
Blotting system
Capillary Electrophoresis
DNA Fragment Size Markers
DNA Gel Extraction Kits
Electrophoresis
Electrophoresis sequencer
Gel Documentation Systems
Protein Extraction Kits and Reagents
Protein extraction system
Protein Gel staining system
UV Crosslinkers
Western Blotting Transfer Systems
Suitable brands
Frequently asked questions
How does an electrophoresis system work?
The system uses an electric field to enable charged molecules such as DNA, RNA, or proteins to migrate through a gel at different speeds depending on their size, resulting in separation.
What types of electrophoresis systems are there?
Generally, a distinction is made between horizontal and vertical systems. Horizontal systems are typically designed for nucleic acids, while vertical systems are mostly used for proteins.
What should I consider when choosing an electrophoresis system?
Key criteria include the type of molecules to be separated, desired gel orientation, number of samples to be processed simultaneously, and compatibility with existing lab infrastructure.
What maintenance is recommended for electrophoresis systems?
Regular cleaning, checking electrical components, and, if necessary, calibration to ensure consistent separation performance are important.
What are the limitations of gel electrophoresis with these systems?
Separation is limited to certain molecular sizes and can be influenced by gel type and running conditions. For more complex analyses, additional methods may be required.
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