Centrifuges - Ultra centrifuge
Ultracentrifuges generate extremely high centrifugal forces to separate macromolecules in solution. They are mainly used in biochemistry and molecular biology.
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Centrifuges - Ultra centrifuge
Ultracentrifuges generate extremely high centrifugal forces to separate macromolecules in solution. They are mainly used in biochemistry and molecular biology.
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Guide and selection support
Ultracentrifuges are used to separate particles such as lipoproteins, viruses, or other macromolecules through very high rotational speeds and the resulting strong centrifugal forces. They are predominantly used in research laboratories in biology, biochemistry, and molecular biology.
When choosing an ultracentrifuge, factors such as maximum speed, rotor type, vacuum operation, and cooling are crucial to ensure separation performance and sample integrity. Other important criteria are user convenience and compatibility with the sample types to be separated.
LabFinder provides a user-oriented overview of ultracentrifuges, supporting targeted research and purchasing by clearly presenting technical specifications and product-relevant features.
Applications and Benefits
Ultracentrifuges are specialized high-speed centrifuges that generate centrifugal forces of up to about 1 million g. They enable ultracentrifugal separation of dissolved macromolecules such as lipoproteins, viruses, or other biological particles based on their density differences in solution. Ultracentrifugation is mainly used in biology, biochemistry, and molecular biology to separate substances according to sedimentation rate, thus allowing for precise analysis and purification.
Selection Criteria
Important selection criteria include the attainable maximum speed and the resulting centrifugal force. The type and material of the rotor are important for compatibility with sample volume and type. Integrated vacuum operation reduces air friction and enables high rotational speeds. Cooling is also essential to minimize temperature effects on samples. Ease of operation and safety features complete the selection process.
Variants and Measurement Principles
Ultracentrifuges operate based on the principle of sedimentation under very high centrifugal force. There are different rotor types, such as swinging bucket rotors or fixed-angle rotors, which are chosen depending on the application. The high rotation speed requires a specially evacuated chamber (vacuum chamber) to reduce air friction.
Calibration and Maintenance
Regular maintenance is important to ensure safe operating conditions and consistent performance. This includes inspecting and, if necessary, replacing seals, rotors, and vacuum systems, as well as checking the control functions. Calibration specifically checks speed and temperature displays to guarantee reproducible results.
Limitations of Application
Ultracentrifuges are suitable only for suspended particles in liquids and not for solids or gas mixtures. The very high g-forces can damage sensitive molecules, so careful sample preparation and parameter selection are essential. In addition, acquisition, operation, and maintenance involve higher costs.
Search Terms and Related Terms
Ultracentrifuge, high-speed centrifuge, ultracentrifuge, high-performance centrifuge, ultra centrifuges, ultracentrifuge lab equipment, sedimentation, centrifugal force, rotor, macromolecule separation, biochemistry centrifuge, molecular biology separation methods, virus particle separation, lipoprotein isolation
Also known as
high-speed centrifuges
ultracentrifuge
ultra centrifuge
ultracentrifuge devices
ultracentrifuge instruments
ultracentrifuge lab
ultracentrifuge laboratory devices
ultracentrifuge machines
ultracentrifuges
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Chromatography Centrifugal Partition (CPC)
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Frequently asked questions
How does an ultracentrifuge work?
Ultracentrifuges generate extremely high rotational speeds, creating strong centrifugal forces to separate particles in suspension based on their density.
What types of rotors are used in ultracentrifuges?
Common rotor types are swinging bucket rotors and fixed-angle rotors, which are selected depending on the sample and separation goal.
What criteria are important when selecting an ultracentrifuge?
Maximum speed, rotor type, vacuum operation, cooling, ease of use, and compatibility with sample volume are key selection criteria.
In which fields are ultracentrifuges mainly used?
Ultracentrifuges are used in biology, biochemistry, and molecular biology, particularly for separating viruses, lipoproteins, and other macromolecules.
What are the limitations of ultracentrifuge applications?
They are only suitable for liquid suspensions, can damage sensitive molecules due to high forces, and require careful sample preparation as well as increased maintenance and operating effort.
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